IL-4 Protein (AA 25-153)
Aperçu rapide pour IL-4 Protein (AA 25-153) (ABIN6387871)
Antigène
Voir toutes IL-4 (IL4) ProtéinesType de proteíne
Activité biologique
Origine
Source
Application
Pureté
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Attributs du protein
- AA 25-153
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Séquence
- MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
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Purification
- > 95% by SDS-PAGE
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niveau d'endotoxine
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤0.6ng/ml.
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Interleukin 4 (IL4) (AA 25-153) protein (His tag)
IL4 Origine: Cynomolgus Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 4 (IL4) (AA 25-153) protein (His-Avi Tag,Biotin)IL4 Origine: Humain Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 4 (IL4) (AA 23-140) (Active) proteinIL4 Origine: Souris Hôte: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) protein (His-Avi Tag)IL4 Origine: Humain Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 4 (IL4) (AA 25-153) (Active) proteinIL4 Origine: Humain Hôte: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag,AVI tag,Biotin)IL4 Origine: Humain Hôte: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 4 (IL4) protein (His tag)IL4 Origine: Souris Hôte: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag)IL4 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Interleukin 4 (IL4) (AA 63-153) protein (GST tag)IL4 Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 4 (IL4) (AA 25-153) protein (mFc Tag)IL4 Origine: Humain Hôte: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Commentaires
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- IL-4 (IL4) (Interleukin 4 (IL4))
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Autre désignation
- IL-4
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Sujet
- IL4, also known as Interleukin 4 isoform1, is a pleiotropic cytokine that has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of naive helper T-cell to Th2 cells. IL4 is a co-stimulator of DNA-synthesis and is induces the expression of class II MHC molecules on resting B-cells. It also enhances both secretion and cell surface expression of IgE and IgG1. Recombinant human IL4, was expressed in E. coli and purified by using conventional chromatography techniques.
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Poids moléculaire
- 15 kDa (130aa)
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NCBI Accession
- NP_000580
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Pathways
- Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
Antigène
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